The development of gene targeting and animal cloning thechnology makes the site - specific integration of foreign gene become possible 隨著基因打靶技術(shù)和動(dòng)物克隆技術(shù)技術(shù)的發(fā)展,外源基因在高等哺乳動(dòng)物基因組中的定點(diǎn)整合成為了可能。
To determine the site - specific integration of the foreign gene into the chloroplast genome and the level of homogeneity , the three transformation lines of the second selective generation were analysized through pcr ( pcr - southern blot ) analysis using the primers of fmdv vp1 gene and homologous fragment of tobacco chloroplast , respectively 經(jīng)過(guò)兩輪抗生素篩選后,采用fmdvvp1基因的一對(duì)引物和煙草葉綠體同源片段的一對(duì)引物分別對(duì)抗性植株的葉綠體dna進(jìn)行的pcr和pcr - southernblot分析。